Strain JE22606
- CGSC#: 6655
- Strain Designation
Designation Source Preference JE22606 National Institute of Genetics 1 pLC26-6/JA200 Nishimura Y. 2 - Sex(Hfr,F+,F-,or F'): F-
- 13 Mutations:
Mutation Location Certainty Display thr-1 0.01 1 thr-1 araC14 1.52 1 araC14 leuB6(Am) 1.74 1 leuB6(Am) lacY1 7.79 1 lacY1 glnX44(AS) 14.99 1 glnX44(AS) galK2(Oc) 16.99 1 galK2(Oc) galT22 17.01 1 galT22 LAM- 17.40 1 λ- trpE63(del) 28.44 1 ΔtrpE63 recA56 60.80 1 recA56 xylA5 80.34 1 xylA5 mtl-1 81.26 1 mtl-1 thiE1 90.34 1 thiE1 - Comments:
- Genes identified on plasmid: leuA, ftsI. Later feedbac said it did not have leuA.
- Host strain: JA200
- Plasmid: pLC26-6
- Episome/Plasmid: pLC26-6
Plasmid Markers/Mutations: araC14 thr-1 leuB6(Am) lacY1 glnX44(AS) galK2(Oc) galT22 LAM- trpE63(del) recA56 xylA5 mtl-1 thiE1 leuA ftsI murE murF ilvI ilvH mafB serR-?? arl polB mraA--? - References:
- Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Nishimura, Y., Y. Takeda, A. Nishimura, H. Suzuki, M. Inouye, Y. Hirota 1977. Synthetic ColE1 plasmids carrying genes for cell division in Escherichia coli. Plasmid 1:67-77
- Clarke, L., J. Carbon 1979. Selection of specific clones from colony banks by suppression or complementation tests. Methods Enzymol. 68:396-408
- Takeda, Y., A. Nishimura, Y. Nishimura, M. Yamada, S. Yasuda, H. Suzuki, Y. Hirota 1981. Synthetic ColE1 plasmids carrying genes for penicillin-binding protein in Escherichia coli. Plasmid 6:86-98
- Neidhardt, F.C., V. Vaughn, T.A. Phillips, P.L. Bloch 1983. Gene-protein index of Escherichia coli K-12. Microbiol.Rev. 47:231-284
Printable Strain Report