You know the name (or CGSC#) of the strain you are looking for.
You want to query for strains that have multiple mutations/properties, such as a specific mating type (e.g. F+, F-, Hfr), or Point of Origin (for Hfrs), or episome/plasmid.
You can use the Mutations field to enter just a gene symbol if there is a fairly limited number of alleles or strains. If there are many alleles and strains, it may be easier to use the MUTATION form described below.
Use this form for examining the "Strain Uses" list.
The gene you are querying has many alleles to avoid retrieving large numbers of strains carrying different alleles of the gene of interest. Choose from the mutations retrieved those alleles of most interest and there will be a list of the strains carrying this mutant allele at the bottom of the record.
Use this form to specify the type of mutation you want to retrieve (e.g., base pair substitution, deletion, Tn). Select from the Type list presented.
Use this form to enter a gene symbol and find out whether we have a mutant allele of this gene and how many. Follow the link to the Strains.
Use this form to find Tn's or other mutations in a range of "map position in minutes" by using the "Mutation Location" fields.
You are searching for information on a particular Gene, Operon, Origin, F'-insert, etc.).
You want to look for an F-prime in a specific region or a deletion in a specific region. Use the Left Endpoint field to query for approx. position. Or use the Name of the F-prime or a gene that may be part of the Deletion Name and select the appropriate Site Type, (e.g., Type: Deletion Fragment and Name: %lac will retrieve many lac deletions.)
Use the synonym field if you do not retrieve a gene that you expect to see in the database.
MapMinute is a rounding off of Left Endpoint coordinate in minutes.
You can query for all regulatory sites or defective phages or genes or operons or insertion sites, etc. in a given region by using the Left Endpoint range fields.
If you use the Gene Product field, be liberal with the `%' wildcard.
The Submutations field is relevant for F-primes and other plasmids.
The Subsites field is relevant for some plasmids, particularly Clarke and Carbon library pLC's. Select Plasmid as Type when querying this field.
You are trying to locate a gene by its gene product or EC#.
Given the variation in gene product names used in the literature, you may want to use the Name field of this form to retrieve the enzyme or RNA or structural protein or the EC# field for enzymes and follow the links in the Encoded By: field to get to the Gene record.
For use of the Name field be liberal in the use of the wild-card (%)
