E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain pLC16-6

• CGSC#: 13853
• Strain Designation

  Designation	Source	Preference
  pLC16-6		1

  
  
• Sex(Hfr,F+,F-,or F'): F+
• 13 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  araC14	1.52	1	araC14
  leuB6(Am)	1.74	1	leuB6(Am)
  lacY1	7.79	1	lacY1
  glnX44(AS)	14.99	1	glnX44(AS)
  galK2(Oc)	16.99	1	galK2(Oc)
  galT22	17.01	1	galT22
  LAM-	17.40	1	λ-
  trpE63(del)	28.44	1	ΔtrpE63
  recA56	60.80	1	recA56
  xylA5	80.34	1	xylA5
  mtl-1	81.26	1	mtl-1
  thiE1	90.34	1	thiE1

  
  
• Comments:
• Episome/Plasmid: pLC16-6

  Plasmid Markers/Mutations:
  thr-1
  araC14
  leuB6(Am)
  lacY1
  glnX44(AS)
  galK2(Oc)
  galT22
  LAM-
  trpE63(del)
  recA56
  xylA5
  mtl-1
  thiE1
  rrnD
  rrfD
  rrlD
  ileU
  alaU
  rrsD
  aroE

• References:
  • Ikemura, T, M Nomura 1977. Expression of spacer tRNA genes in ribosomal RNA transcription units carried by hybrid Col E1 plasmids in E. coli. Cell 11(4):779-93.
  • Kenerley, M.E., E.A. Morgan, L. Post, L. Lindahl, M. Nomura 1977. Characterization of hybrid plasmids carrying individual ribosomal ribonucleic acid transcription units of Escherichia coli. J.Bacteriol. 132:931-949
  • Clarke, L., J. Carbon 1979. Selection of specific clones from colony banks by suppression or complementation tests. Methods Enzymol. 68:396-408
  • Neidhardt, F.C., V. Vaughn, T.A. Phillips, P.L. Bloch 1983. Gene-protein index of Escherichia coli K-12. Microbiol.Rev. 47:231-284
  
  

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