Strain pLC25-33
- CGSC#: 13780
- Strain Designation
Designation Source Preference pLC25-33 1 - Sex(Hfr,F+,F-,or F'): F+
- 13 Mutations:
Mutation Location Certainty Display thr-1 0.01 1 thr-1 araC14 1.52 1 araC14 leuB6(Am) 1.74 1 leuB6(Am) lacY1 7.79 1 lacY1 glnX44(AS) 14.99 1 glnX44(AS) galK2(Oc) 16.99 1 galK2(Oc) galT22 17.01 1 galT22 LAM- 17.40 1 λ- trpE63(del) 28.44 1 ΔtrpE63 recA56 60.80 1 recA56 xylA5 80.34 1 xylA5 mtl-1 81.26 1 mtl-1 thiE1 90.34 1 thiE1 - Comments:
- Episome/Plasmid: pLC25-33
Plasmid Markers/Mutations: araC14 thr-1 leuB6(Am) lacY1 glnX44(AS) galK2(Oc) galT22 LAM- trpE63(del) recA56 xylA5 mtl-1 thiE1 melA melB fumB - References:
- Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Tsuchiya, T, K Ottina, Y Moriyama, MJ Newman, TH Wilson 1982. Solubilization and reconstitution of the melibiose carrier from a plasmid-carrying strain of Escherichia coli. J. Biol. Chem. 257(9):5125-8.
- Guest, J.R., J.S. Miles, R.E. Roberts, S.A. Woods 1985. The fumarase genes of Escherichia coli: location of the fumB gene and discovery of a new gene (fumC). J.Gen.Microbiol. 131:2971-2984
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151
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