Strain - BW25113/pKD46/pB15

Strain - BW25113/pKD46/pB15Strain BW25113/pKD46/pB15

Strain BW25113/pKD46/pB15

CGSC#: 14236
Strain Designation
Designation Source Preference
BW25113/pKD46/pB15 Wertz J.E. 1
Sex(Hfr,F+,F-,or F'): F-

Designation	Source	Preference
BW25113/pKD46/pB15	Wertz J.E.	1

6 Mutations:

Mutation	Location	Certainty	Display
DE(araD-araB)567	1.40	1	Δ(araD-araB)567
lacZ4787(del)(::rrnB-3)	7.81	1	ΔlacZ4787(::rrnB-3)
LAM-	17.40	1	λ^-
rph-1	82.20	1	rph-1
DE(rhaD-rhaB)568	88.16	1	Δ(rhaD-rhaB)568
hsdR514	98.74	1	hsdR514

Comments:
- pKD46 is more efficient than pKD20 for making gene disruptions. pKD46 sequence: GenBank AY048746. See also pKD78(CmR) and pKD119(TetR).
- The ara and rha deletions were published as Δ rhaBAD_LD78 and Δ araBAD_AH33
- This strain also contains the conjugative pB15 plasmid from PET297
- This strain should be grown on rich media with ampicillin (100 ug/ml at 30 C).
Episome/Plasmid: pKD46
Plasmid Markers/Mutations:
repA101(ts)
araBp-gam-bet-exo
oriR101
bla(ApR)
araC
bla
[tL3]
References:
- Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.
- Turner, PE, ES Williams, C Okeke, VS Cooper, S Duffy, JE Wertz 2014. Antibiotic resistance correlates with transmission in plasmid evolution. Evolution 68(12):3368-80.
To request this strain visit the ECGRC website.

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