E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Strain TA531

• CGSC#: 12283
• Strain Designation

  Designation	Source	Preference
  TA531	Squires C.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 12 Mutations:

  Mutation	Location	Certainty	Display
  ara-600	1.40	1	ara-600
  DE(rrsH-rrlH)770	4.82	1	Δ(rrsH-rrlH)770
  DE(codB-lacI)3	7.60	1	Δ(codB-lacI)3
  DE(rrlG-rrsG)772::lacZ	58.72	1	Δ(rrlG-rrsG)772::lacZ
  DE(recA-srl)306	60.79	1	Δ(recA-srl)306
  srlR301::Tn10	60.94	1	srlR301::Tn10
  DE(rrlD-rrsD)773::cat	73.75	1	Δ(rrlD-rrsD)773::cat
  DE(rrsC-rrlC)774::cat	84.96	1	Δ(rrsC-rrlC)774::cat
  DE(rrsA-rrlA)771	86.93	1	Δ(rrsA-rrlA)771
  DE(rrsB-rrlB)769	89.76	1	Δ(rrsB-rrlB)769
  thiE1	90.34	0	thiE1
  DE(purD-metA)768	90.58	1	Δ(purD-metA)768

  
  
• Comments:
• Episome/Plasmid: ptRNA66

  Plasmid Markers/Mutations:
  aadA(strR,SpcR)
  ori p15A
  trpT
  aspT
  ileU
  alaU
  thrV

• Episome/Plasmid: pHK-rrnC

  Plasmid Markers/Mutations:
  kan
  oriR101
  trpT
  rrsC
  rrlC
  rrfC
  gltU
  aspT

• References:
  • Asai, T, D Zaporojets, C Squires, CL Squires 1999. An Escherichia coli strain with all chromosomal rRNA operons inactivated: complete exchange of rRNA genes between bacteria. Proc. Natl. Acad. Sci. U.S.A. 96(5):1971-6.
  
  

  Printable Strain Report