CGSC Strain#: 12283
Strain Designation: TA531      Source of Strain: C.L. Squires
Sex: F- Episome/Plasmid: ptRNA66
   Plasmid Markers/Mutations: aadA(strR,SpcR), ori_p15A, trpT , aspT , ileU , alaU , thrV
Episome/Plasmid: pHK-rrnC
   Plasmid Markers/Mutations: kan, oriR101, trpT , rrsC , rrlC , rrfC , gltU , aspT
Chromosomal Markers: ara-600, Δ(rrsH-rrlH)770, Δ(codB-lacI)3, Δ(rrlG-rrsG)772::lacZ, Δ(recA-srl)306, srlR301::Tn10, Δ(rrlD-rrsD)773::cat, Δ(rrsC-rrlC)774::cat , Δ(rrsA-rrlA)771, Δ(rrsB-rrlB)769, thiE1, Δ(purD-metA)768
Strain Comments:

• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(recA-srl)306-- removes all, or nearly all, of the recA gene and extends into srlR, but does not remove the Tn10 insertion in srlR
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(purD-metA)768 was formerly called Δ(purDH-rrnE-metA)
• Δ(rrlD-rrsD)773::cat was formerly called Δ(rrsD-ileU-alaU-rrlD)25::cat
• Δ(rrlG-rrsG)772::lacZ was formerly called Δ(rrsG-gltW-rrlG)30::lacZ
• Δ(rrsA-rrlA)771 was formerly called Δ(rrsA-ileT-alaT-rrlA)34
• Δ(rrsB-rrlB)769 was formerly called Δ(rrsB-gltT-rrlB)101
• Δ(rrsC-rrlC)774::cat was formerly called Δ(rrsC-gltU-rrlC)15::cat
• Δ(rrsH-rrlH)770 was formerly called Δ(rrsH-ileV-alaV-rrlH)103
• thiE1 was formerly called thi-1
• = chloramphenicol resistant
• = tetracycline resistant
• SpcR = spectinomycin resistant
• strR = streptomycin resist.
• = kanamycin resistant

References:

• Asai, T, D Zaporojets, C Squires, CL Squires 1999. An Escherichia coli strain with all chromosomal rRNA operons inactivated: complete exchange of rRNA genes between bacteria. Proc. Natl. Acad. Sci. U.S.A. 96(5):1971-6.