E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Site ptRNA66

• Name: ptRNA66
• Type: Plasmid
  SubSites:

  trpT

  aspT

  ileU

  alaU

  thrV

  
  
  Plasmid Sites:

  aadA(strR,SpcR)

  ori p15A

  
  
• Priority: 0
• Comment:
• References:
  • Asai, T, D Zaporojets, C Squires, CL Squires 1999. An Escherichia coli strain with all chromosomal rRNA operons inactivated: complete exchange of rRNA genes between bacteria. Proc. Natl. Acad. Sci. U.S.A. 96(5):1971-6.
  
  
• 2 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  TA527	10	F-, ara-600, Δ(rrsH-rrlH)770, Δ(codB-lacI)3, Δ(rrlG-rrsG)772::lacZ, Δ(rrlD-rrsD)773::cat, Δ(rrsC-rrlC)774::cat , Δ(rrsA-rrlA)771, Δ(rrsB-rrlB)769, thiE1?, Δ(purD-metA)768, ptRNA66, pHK-rrnC
  TA531	12	F-, ara-600, Δ(rrsH-rrlH)770, Δ(codB-lacI)3, Δ(rrlG-rrsG)772::lacZ, Δ(recA-srl)306, srlR301::Tn10, Δ(rrlD-rrsD)773::cat, Δ(rrsC-rrlC)774::cat , Δ(rrsA-rrlA)771, Δ(rrsB-rrlB)769, thiE1?, Δ(purD-metA)768, ptRNA66, pHK-rrnC