Site pLC34-20B
- Name: pLC34-20B
- Type: Plasmid
- SubSites:
pyrH tdi-?? dnaE proS metD fadE rrnH rrsH alaV ileV rrlH rrfH aspU dnaQ rnhA strC - Plasmid Sites:
thr-1 araC14 leuB6(Am) lacY1 glnX44(AS) galK2(Oc) galT22 LAM- trpE63(del) recA56 xylA5 mtl-1 thiE1 - Priority: 0
- Comment:
- Assumed to have all genes between dnaE and dnaQ. Hybridizes with Kohara clones 121-123. Carries strC ca. 7.0 minutes. See also pLC34-20A.
- References:
- Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Clarke, L., J. Carbon 1979. Selection of specific clones from colony banks by suppression or complementation tests. Methods Enzymol. 68:396-408
- Nishijima, M., C.E. Bulawa, C.H.R. Raetz 1981. Two interacting mutations causing temperature-sensitive phosphatidylglycerol synthesis in Escherichia coli membranes. J.Bacteriol. 145:113-131
- Horiuchi, T., H. Maki, M. Maruyana, M. Sekiguchi 1981. Identification of the dnaQ gene product and location of the structural gene for RNase H of Escherichia coli by cloning of the genes. Proc.Natl.Acad.Sci.USA 78:3770-3774
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151
- 1 Strains Carrying This Plasmid
Name Mutations Genotype pLC34-20B 13 F+, thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1, pLC34-20B