Site pLC4-6D
- Name: pLC4-6D
- Type: Plasmid
- SubSites:
cysB trpB trpC trpD trpE topA - Plasmid Sites:
thr-1 araC14 leuB6(Am) lacY1 glnX44(AS) galK2(Oc) galT22 LAM- trpE63(del) recA56 xylA5 mtl-1 thiE1 - Priority: 0
- Comment:
- Mixed clone. See also pLC4-6A, plC4-6B, and pLC4-6C. Hybridizes with Kohara clones 252-254, 119, 146, and 450. Same hybridization pattern as pLC5-23.
- References:
- Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Sternglanz, R., S. DiNardo, K.A. Voelkel, Y. Nishimura, Y. Hirota, K. Becherer, L. Zumstein, J.C. Wang 1981. Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and translation. Proc.Natl.Acad.Sci.USA 78:2747-2751
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151
- 1 Strains Carrying This Plasmid
Name Mutations Genotype pLC4-6D 13 F+, thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1, pLC4-6D