E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain DF264

• CGSC#: 6381
• Strain Designation

  Designation	Source	Preference
  DF264	Fraenkel D.	1

  
  
• Sex(Hfr,F+,F-,or F'): Hfr
• Hfr Point of Origin#: 2A
• PO Map Position: 12.20
• Direction of Marker Transfer: Counterclockwise
• 12 Mutations:

  Mutation	Location	Certainty	Display
  garB10	3.60	1	garB10
  fhuA22	3.61	1	fhuA22
  ompF627(T2R)	21.24	1	ompF627(T2R)
  fadL701(T2R)	53.01	1	fadL701(T2R)
  relA1	62.71	1	relA1
  pgk-2	66.16	1	pgk-2
  speA210::Tn10	66.43	1	speA210::Tn10
  pitA10	78.36	1	pitA10
  spoT1	82.34	1	spoT1
  rrnB-2	89.76	1	rrnB-2
  mcrB1	98.63	1	mcrB1
  creC510	99.89	1	creC510

  
  
• Comments:
  • This strain should not be grown on a medium containing glucose because glucose will select for revertants of pgk-2. It can be grown on minimal medium + glycerol + malate or succinate.
• References:
  • Thomson, J, PD Gerstenberger, DE Goldberg, E Gociar, A Orozco de Silva, DG Fraenkel 1979. ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt. J. Bacteriol. 137(1):502-6.
  
  

  Printable Strain Report