CGSC Strain#: 6381
Strain Designation: DF264      Source of Strain: D. Fraenkel
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, ompF627(T2R), fadL701(T2R), relA1, pgk-2, speA210::Tn10, pitA10, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• This strain should not be grown on a medium containing glucose because glucose will select for revertants of pgk-2. It can be grown on minimal medium + glycerol + malate or succinate.
• pgk-2-- : Glucose will select for revertants of pgk-2. (Mutations in the eno, gap, and pgk genes all revert and must be monitored closely.)
• pgk-2-- Does not allow growth on glycerol alone or oxidative substrate alone; cannot grow on sugars; glucose inhibits growth on other substrates. Can grow on glycerol plus oxidative substrate (malate)
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• pgk-2 was formerly called pgk-1
• speA210::Tn10 was formerly called zgf-210::Tn10
• = tetracycline resistant
• Const = constitutive
• T2R = T2 Resistant

References:

• Thomson, J, PD Gerstenberger, DE Goldberg, E Gociar, A Orozco de Silva, DG Fraenkel 1979. ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt. J. Bacteriol. 137(1):502-6.