E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation pgk-2

• Name: pgk-2
• Type: Unknown
• Mutation of Gene: pgk
• Approx. Map Location: 66.16
• Synonyms

  Designation	Where Used
  pgk-1

  
  
• Comments
  • : Glucose will select for revertants of pgk-2. (Mutations in the eno, gap, and pgk genes all revert and must be monitored closely.)
  • Does not allow growth on glycerol alone or oxidative substrate alone; cannot grow on sugars; glucose inhibits growth on other substrates. Can grow on glycerol plus oxidative substrate (malate)
• References
  • Thomson, J, PD Gerstenberger, DE Goldberg, E Gociar, A Orozco de Silva, DG Fraenkel 1979. ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt. J. Bacteriol. 137(1):502-6.
• 3 Strains Carrying This Mutation

  Name	Mutations	Genotype
  DF576	10	F-, glnX44(AS), λ-, relA1, pgk-2, rpsL104, malA?, malT1(λR)?, xyl-7?, mtlA2?, thiE1?
  DF263	11	Hfr(PO2A), garB10, fhuA22, ompF627(T2R), fadL701(T2R), relA1, pgk-2, pitA10, spoT1, rrnB-2, mcrB1, creC510
  DF264	12	Hfr(PO2A), garB10, fhuA22, ompF627(T2R), fadL701(T2R), relA1, pgk-2, speA210::Tn10, pitA10, spoT1, rrnB-2, mcrB1, creC510