E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Strain KL664

• CGSC#: 6215
• Strain Designation

  Designation	Source	Preference
  KL664	Cappello M.C.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 10 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  serR-??-35	2.30	1	serR-??-35
  DE(gpt-proA)62	5.51	1	Δ(gpt-proA)62
  glnX44(AS)	14.99	1	glnX44(AS)
  serS15(ts)	20.23	1	serS15(ts)
  rpsL31(strR)	74.84	1	rpsL31(strR)
  xylA5	80.34	1	xylA5
  mtl-1	81.26	1	mtl-1
  argE3(Oc)	89.48	1	argE3(Oc)
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • This strain is temperature-resistant at 42 degrees and temperature-sensitive at 45 degrees C on Luria agar.
• References:
  • Theall, G., K.B. Low, D. Soll 1979. Regulation of the biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. IV. Mutants with increased levels of leucyl- or seryl-tRNA synthetase. Mol.Gen.Genet. 169:205-211
  
  

  Printable Strain Report