CGSC Strain#: 6215
Strain Designation: KL664      Source of Strain: M.C. Cappello
Sex: F- Chromosomal Markers: thr-1, serR-??-35, Δ(gpt-proA)62, glnX44(AS), serS15(ts), rpsL31(strR), xylA5, mtl-1, argE3(Oc), thiE1
Strain Comments:

• This strain is temperature-resistant at 42 degrees and temperature-sensitive at 45 degrees C on Luria agar.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• serS15(ts)-- is a temperature-sensitive mutation.
• Δ(gpt-proA)62 was formerly called proA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Theall, G., K.B. Low, D. Soll 1979. Regulation of the biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. IV. Mutants with increased levels of leucyl- or seryl-tRNA synthetase. Mol.Gen.Genet. 169:205-211