E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Strain BS40dnaE74cydA85

• CGSC#: 8168
• Strain Designation

  Designation	Source	Preference
  BS40dnaE74cydA85	Strauss B.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 12 Mutations:

  Mutation	Location	Certainty	Display
  dnaE74(ts)	4.42	1	dnaE74(ts)
  pro-3(del)	5.92	0	Δpro-3
  DE(gpt-lac)5 or DE(cod-lacI)6	8.00	1	Δ(gpt-lac)5 or Δ(cod-lacI)6
  tsx-76	9.28	1	tsx-76
  cydA85	16.61	1	cydA85
  nadA57::Tn10	16.84	1	nadA57::Tn10
  LAM-	17.40	1	λ-
  relA1	62.71	0	relA1
  rpsL-(strR)	74.84	1	rpsL-(strR)
  spoT1	82.34	0	spoT1
  metB1	88.94	1	metB1
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • The cross used in constructing this strain either brings in the pro-3 and Δ (cod-lacI)6 deletion from CD4 or leaves the Δ (gpt-lac)5, aka (pro-lac), deletion of the S90C parent.
• References:
  • Strauss, B., K. Kelly, T. Dincman, D. Ekiert, T. Biesieda, R. Song 2004. Cell death in Escherichia coli dnaE(Ts) mutants incubated at a nonpermissive temperature is prevented by mutation in the cydA gene. J.Bacteriol. 186:2147-2155
  
  

  Printable Strain Report