Mutation dnaE74(ts)
- Name: dnaE74(ts)
- Type: Base Pair Substitution
- Mutation of Gene: dnaE
- Approx. Map Location: 4.42
- Properties
Property Symbol Temperature Sensitive ts - Comments
- causes cell death after 2 hours or more at 40C (unless suppressed)
- References
- Brotcorne-Lannoye, A, G Maenhaut-Michel, M Radman 1985. Involvement of DNA polymerase III in UV-induced mutagenesis of bacteriophage lambda. Mol. Gen. Genet. 199(1):64-9.
- Strauss, B., K. Kelly, T. Dincman, D. Ekiert, T. Biesieda, R. Song 2004. Cell death in Escherichia coli dnaE(Ts) mutants incubated at a nonpermissive temperature is prevented by mutation in the cydA gene. J.Bacteriol. 186:2147-2155
- 1 Strains Carrying This Mutation
Name Mutations Genotype BS40dnaE74cydA85 12 F-, dnaE74(ts), Δpro-3?, Δ(gpt-lac)5 or Δ(cod-lacI)6, tsx-76, cydA85, nadA57::Tn10, λ-, relA1?, rpsL-(strR), spoT1?, metB1, thiE1