E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation cydA85

• Name: cydA85
• Type: Base Pair Substitution
• Mutation of Gene: cydA
• Approx. Map Location: 16.61
• Comments
  • : C to T mutation at base position 770934. It suppresses the temperature sensitive cell death in dnaE74 cells.
• References
  • Strauss, B., K. Kelly, T. Dincman, D. Ekiert, T. Biesieda, R. Song 2004. Cell death in Escherichia coli dnaE(Ts) mutants incubated at a nonpermissive temperature is prevented by mutation in the cydA gene. J.Bacteriol. 186:2147-2155
• 2 Strains Carrying This Mutation

  Name	Mutations	Genotype
  BS40cydA85	11	F-, Δpro-3?, Δ(gpt-lac)5 or Δ(cod-lacI)6, tsx-76, cydA85, nadA57::Tn10, λ-, relA1?, rpsL-(strR), spoT1?, metB1, thiE1
  BS40dnaE74cydA85	12	F-, dnaE74(ts), Δpro-3?, Δ(gpt-lac)5 or Δ(cod-lacI)6, tsx-76, cydA85, nadA57::Tn10, λ-, relA1?, rpsL-(strR), spoT1?, metB1, thiE1