E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Strain EP432

• CGSC#: 7974
• Strain Designation

  Designation	Source	Preference
  EP432	Schuldiner S.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 7 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  nhaA1(del)::kan	0.38	1	ΔnhaA1::kan
  DE(lacY-lacZ)9998	7.77	1	Δ(lacY-lacZ)9998
  nhaB3(del)::Cm	26.57	1	ΔnhaB3::Cm
  rpsL-(strR)	74.84	1	rpsL-(strR)
  melB-(ts,LiD)	93.57	1	melB-(ts,LiD)
  zjd-2334::Tn10	93.70	1	zjd-2334::Tn10

  
  
• Comments:
  • This strain is sensitive to Na+ and should be grown on LBK medium(LB plus KCl (6.5 g/l) lacking NaCl).
• References:
  • Pinner, E., Y. Kotler, E. Padan, S. Schuldiner 1993. Physiological role of nhaB, a specific Na+/H+ antiporter in Escherichia coli. J.Biol.Chem. 268:1729-1734
  
  

  Printable Strain Report