CGSC Strain#: 7974
Strain Designation: EP432      Source of Strain: S. Schuldiner
Sex: F- Chromosomal Markers: thr-1, ΔnhaA1::kan, Δ(lacY-lacZ)9998, ΔnhaB3::Cm, rpsL-(strR), melB-(ts,LiD), zjd-2334::Tn10
Strain Comments:

• This strain is sensitive to Na+ and should be grown on LBK medium(LB plus KCl (6.5 g/l) lacking NaCl).
• melB-(ts,LiD)-- allows growth on melibiose at 30 C, but not at 37 C; after several days at 37 C, spontaneous revertants will arise.
• ΔnhaB3::Cm was formerly called nhaB1
• = kanamycin resistant
• = tetracycline resistant
• LiD = lithium dependent
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Pinner, E., Y. Kotler, E. Padan, S. Schuldiner 1993. Physiological role of nhaB, a specific Na+/H+ antiporter in Escherichia coli. J.Biol.Chem. 268:1729-1734