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CGSC, The Coli Genetic Stock Center

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Mutation melB-(ts,LiD)

• Name: melB-(ts,LiD)
• Type: Unknown
• Approx. Map Location: 93.57
• Properties

  Property	Symbol
  lithium dependent	LiD
  Temperature Sensitive	ts

  
  
• Comments
  • allows growth on melibiose at 30 C, but not at 37 C; after several days at 37 C, spontaneous revertants will arise.
• References
  • Lopilato, J., T. Tsuchiya, T.H. Wilson 1978. Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli. J.Bacteriol. 134:147-156
• 4 Strains Carrying This Mutation

  Name	Mutations	Genotype
  TA15	4	F-, Δ(lacY-lacZ)9998, rpsL-(strR), melB-(ts,LiD), zjd-2334::Tn10
  EP431	5	F-, Δ(lacY-lacZ)9998, ΔnhaB3::Cm, rpsL-(strR), melB-(ts,LiD), zjd-2334::Tn10
  NM81	6	F-, thr-1, ΔnhaA1::kan, Δ(lacY-lacZ)9998, rpsL-(strR), melB-(ts,LiD), zjd-2334::Tn10
  EP432	7	F-, thr-1, ΔnhaA1::kan, Δ(lacY-lacZ)9998, ΔnhaB3::Cm, rpsL-(strR), melB-(ts,LiD), zjd-2334::Tn10