Strain JM109/pWM5
- CGSC#: 8267
- Strain Designation
Designation Source Preference JM109/pWM5 Wanner B.L. 1 - Sex(Hfr,F+,F-,or F'): F'
- 10 Mutations:
Mutation Location Certainty Display DE(gpt-lac)0 5.51 1 Δ(gpt-lac)0 glnX44(AS) 14.99 1 glnX44(AS) LAM- 17.40 1 λ- rfbC1 45.43 0 rfbC1 gyrA96(NalR) 50.33 1 gyrA96(NalR) recA1 60.80 1 recA1 endA1 66.57 1 endA1 spoT1 82.34 0 spoT1 thiE1 90.34 1 thiE1 hsdR17 98.74 1 hsdR17 - Comments:
- Episome/Plasmid: pWM5
Plasmid Markers/Mutations: bla(ApR) aadA(strR,SpcR) uidA(p-) MCS-2 - Episome/Plasmid: F128-x
Plasmid Markers/Mutations: lacIp-4000(lacIQ) lacZ58(del)(M15) traD36 proA proB - References:
- Yanisch-Perron, C, J Vieira, J Messing 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33(1):103-19.
- Metcalf, W.W., B.L. Wanner 1993. Construction of new beta-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement. Gene 129:17-25
Printable Strain Report