E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation lacZ58(del)(M15)

• Name: lacZ58(del)(M15)
• Type: Deletion
• Mutation of Gene: lacZ
• Approx. Map Location: 7.81
• Synonyms

  Designation	Where Used
  deletion M15	Beckwith
  lacZ(M15)
  M15 deletion	Beckwith

  
  
• Properties

  Property	Symbol
  alpha-complemented allele	M15
  deletion	del

  
  
• Comments
  • is a deletion of a small part (the alpha region) of the lacZ gene; allows `alpha complementation'.
• References
  • BECKWITH, JR 1964. A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI. J. Mol. Biol. 8:427-30.
• 4 Strains Carrying This Mutation

  Name	Mutations	Genotype
  RC30	7	Hfr(PO1), ΔlacZ58(M15), lacI22, λ-, e14-, relA1, spoT1, thiE1
  BW29427	8	F-, RP4-2(TetS, kan1360::FRT)?, thrB1004?, ΔlacZ58(M15)?, ΔdapA1341::[erm pir+]?, rpsL(strR)?, thi-?, hsdS-?, pro-?
  XA21	8	F-, ΔlacZ58(M15), lacI22, λ-, rpsL135(strR), malT1(λR), xyl-7, mtlA2, thiE1
  K1342	14	Hfr(PO2A), garB10, fhuA22, ΔlacZ58(M15), lacIp-4000(lacIQ)?, mhpC281::Tn10, phoA4(Am), ompF627(T2R), fadL701(T2R), relA1, pitA10, spoT1, rrnB-2, mcrB1, creC510