CGSC Strain#: 8267
Strain Designation: JM109/pWM5      Source of Strain: B.L. Wanner
Sex: F' Episome/Plasmid: pWM5
   Plasmid Markers/Mutations: bla(ApR), aadA(strR,SpcR), uidA(p-), MCS-2
Episome/Plasmid: F128-x
   Plasmid Markers/Mutations: lacIp-4000(lacI^Q), ΔlacZ58(M15), traD36, proA , proB
   Plasmid Comment: an F128-like F' found in Messing strains and their derivatives
Chromosomal Markers: Δ(gpt-lac)0, glnX44(AS), λ^-, rfbC1, gyrA96(NalR), recA1, endA1, spoT1, thiE1, hsdR17
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• gyrA96(NalR)-- GAC to AAC at codon 87
• gyrA96(NalR)-- resistant to 200ug/ml nalidixic acid
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• gyrA96(NalR) was formerly called
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.
• ApR = ampicillin resistant
• SpcR = spectinomycin resistant
• strR = streptomycin resist.
• M15 = alpha-complemented allele

References:

• Yanisch-Perron, C, J Vieira, J Messing 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33(1):103-19.
• Metcalf, W.W., B.L. Wanner 1993. Construction of new beta-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement. Gene 129:17-25