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CGSC, The Coli Genetic Stock Center

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Strain BW22245/pWM71

• CGSC#: 8214
• Strain Designation

  Designation	Source	Preference
  BW22245/pWM71	Wanner B.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 7 Mutations:

  Mutation	Location	Certainty	Display
  DE(codB-lacI)3	7.60	1	Δ(codB-lacI)3
  phoA532(del)	8.64	1	ΔphoA532
  uidA4(del)::pir-116	36.48	1	ΔuidA4::pir-116
  recA1	60.80	1	recA1
  rpoS396(Am)	61.75	0	rpoS396(Am)
  rph-1	82.20	1	rph-1
  DE(phnP-phnD)3330( phnC?)	92.93	1	Δ(phnP-phnD)3330( phnC?)

  
  
• Comments:
  • pWM71 is like pAH34 except for PstI site in bla. pAH34 host strain is pir⁺ and F^-.
• Episome/Plasmid: F128-Tn10-11

  Plasmid Markers/Mutations:
  lacIp-4000(lacIQ)
  lacZ58(del)(M15)
  F::Tn10-11
  proA
  proB
  Tn10-11
  lac

• Episome/Plasmid: pWM71

  Plasmid Markers/Mutations:
  oriR6Kgamma
  bla(ApR)
  MCS
  oriT RP4
  ori f1,+
  lacZ alpha
  proA
  proB
  Tn10-11

• References:
  • Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
  
  

  Printable Strain Report