Site pWM71
- Name: pWM71
- Type: Plasmid
- SubSites:
proA proB Tn10-11 - Plasmid Sites:
oriR6Kgamma bla(ApR) MCS oriT RP4 ori f1,+ lacZ alpha - Priority: 0
- Comment:
- MCS polylinker sites ApaI, BamHI, ClaI, EcoRV, KpnI, NotI, SacI, SacII, SalI, SmaI, SpeI, XbaI, XhoI. f1 ori in (+) orientation; {\it lacZ$\alpha$-ori$_f1} from pBlueScriptII(SK+) . See pAH34
- References:
- Alting-Mees, M.A., J.A. Sorge, J.M. Short 1992. pBluescriptII: Multifunctional cloning and mapping vectors. Methods Enzymol. 216:483-495
- Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
- 1 Strains Carrying This Plasmid
Name Mutations Genotype BW22245/pWM71 7 F-, Δ(codB-lacI)3, ΔphoA532, ΔuidA4::pir-116, recA1, rpoS396(Am)?, rph-1, Δ(phnP-phnD)3330( phnC?), F128-Tn10-11, pWM71