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Mutation uidA4(del)::pir-116

• Name: uidA4(del)::pir-116
• Type: Insertion
• Mutation of Gene: uidA
• Approx. Map Location: 36.48
• Synonyms

  Designation	Where Used
  uidA(Delta MluI::pir+

  
  
• Properties

  Property	Symbol
  deletion	del

  
  
• Comments
  • Allows plasmids with an R6Kγ origin to replicate to high copy number
• References
  • Haldimann, A, BL Wanner 2001. Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria. J. Bacteriol. 183(21):6384-93.
• 20 Strains Carrying This Mutation

  Name	Mutations	Genotype
  BW18813/pBW30	7	F-, Δ(codB-lacI)3, ΔphoA532, ΔuidA4::pir-116, rpoS396(Am)?, rph-1, Δ(phnP-phnD)3330( phnC?), [phnB], pBW30
  BW20338	7	F', Δ(codB-lacI)3, ΔphoA532, ΔuidA4::pir-116, recA1, rpoS396(Am)?, rph-1, Δ(phnP-phnD)3330( phnC?), F128-Tn10-12
  BW21037	7	F-, Δ(codB-lacI)3, ΔphoA532, ΔuidA4::pir-116, recA1, rph-1, Δ(phnP-phnD)3330( phnC?), mini-Tn5Lac4(CamR,lacIQ)
  BW22245	7	F', Δ(codB-lacI)3, ΔphoA532, ΔuidA4::pir-116, recA1, rpoS396(Am)?, rph-1, Δ(phnP-phnD)3330( phnC?), F128-Tn10-11
  BW22245/pWM71	7	F-, Δ(codB-lacI)3, ΔphoA532, ΔuidA4::pir-116, recA1, rpoS396(Am)?, rph-1, Δ(phnP-phnD)3330( phnC?), F128-Tn10-11, pWM71
  BW23322	8	F-, Δ(argF-lac)169, ΔuidA4::pir-116, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510
  BW23474	9	F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510
  BW23474/pAH120	9	F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510, pAH120
  BW23474/pAH143	9	F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510, pAH143
  BW23474/pAH144	9	F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510, pAH144
  BW23474/pAH145	9	F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT?, rph-1, hsdR514, rob-1, creC510, pAH145
  BW23474/pAH152	9	F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510, , pAH152
  BW23474/pAH153	9	F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510, pAH153
  BW23474/pAH154	9	F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510, pAH154
  BW23474/pAH156	9	F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510, pAH156
  BW23474/pAH68	9	F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510, pAH68
  BW23474/pAH70	9	F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510, pAH70
  BW23474/pAH81	9	F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510, pAH81
  BW23474/pAH95	9	F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am)?, endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510, pAH95
  BW25142	11	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA4::pir-116, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514