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Strain BW25113/pKD46

• CGSC#: 7739
• Strain Designation

  Designation	Source	Preference
  BW25113/pKD46	Wanner B.L.	1
  pKD46		2

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 6 Mutations:

  Mutation	Location	Certainty	Display
  DE(araD-araB)567	1.40	1	Δ(araD-araB)567
  lacZ4787(del)(::rrnB-3)	7.81	1	ΔlacZ4787(::rrnB-3)
  LAM-	17.40	1	λ-
  rph-1	82.20	1	rph-1
  DE(rhaD-rhaB)568	88.16	1	Δ(rhaD-rhaB)568
  hsdR514	98.74	1	hsdR514

  
  
• Comments:
  • pKD46 is more efficient than pKD20 for making gene disruptions. pKD46 sequence: GenBank AY048746. See also pKD78(CmR) and pKD119(TetR).
  • The ara and rha deletions were published as Δ rhaBAD_LD78 and Δ araBAD_AH33
  • This strain should be grown on rich media with ampicillin (100 ug/ml at 30 C).
• Episome/Plasmid: pKD46

  Plasmid Markers/Mutations:
  repA101(ts)
  araBp-gam-bet-exo
  oriR101
  bla(ApR)
  araC
  bla
  [tL3]

• References:
  • Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.
  
  

  Printable Strain Report