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Strain NH4920

• CGSC#: 5041
• Strain Designation

  Designation	Source	Preference
  NH4920	Hall J.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 17 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  araC14	1.52	0	araC14
  leuB6(Am)	1.74	1	leuB6(Am)
  DE(gpt-proA)62	5.51	1	Δ(gpt-proA)62
  lacY1	7.79	1	lacY1
  tsx-33	9.28	1	tsx-33
  glnX44(AS)	14.99	0	glnX44(AS)
  galK2(Oc)	16.99	1	galK2(Oc)
  LAM-	17.40	1	λ-
  hisG4(Oc)	45.01	1	hisG4(Oc)
  recA44(ts)	60.80	1	recA44(ts)
  rpsL31(strR)	74.84	1	rpsL31(strR)
  xylA5	80.34	1	xylA5
  mtl-1	81.26	0	mtl-1
  argE3(Oc)	89.48	1	argE3(Oc)
  thiE1	90.34	1	thiE1
  deoB16	99.53	1	deoB16

  
  
• Comments:
  • The strain does not grow well at high temperature. Use 42 degrees as the restrictive and 32 degrees as the permissive temp.
• References:
  • Hall, JD, P Howard-Flanders 1975. Temperature-sensitive recA mutant of Escherichia coli K-12: deoxyribonucleic acid metabolism after ultraviolet irradiation. J. Bacteriol. 121(3):892-900.
  
  

  Printable Strain Report