Strain CJ376
- CGSC#: 12519
- Strain Designation
Designation Source Preference CJ376 Joyce C.M. 1 CJ376/pCJ136 2 - Sex(Hfr,F+,F-,or F'): F-
- 21 Mutations:
Mutation Location Certainty Display thr-1 0.01 1 thr-1 araC14 1.52 1 araC14 leuB6(Am) 1.74 1 leuB6(Am) DE(gpt-proA)62 5.51 1 Δ(gpt-proA)62 lacY1 7.79 1 lacY1 tsx-33 9.28 1 tsx-33 qsr'-0 12.15 1 qsr'-0 glnX44(AS) 14.99 1 glnX44(AS) galK2(Oc) 16.99 1 galK2(Oc) LAM- 17.40 1 λ- Rac-0 30.39 1 Rac-0 DE(xthA-pncA)90 39.45 1 Δ(xthA-pncA)90 hisG4(Oc) 45.01 1 hisG4(Oc) rfbC1 45.43 1 rfbC1 recA1925 60.80 1 recA1925 rpsL31(strR) 74.84 1 rpsL31(strR) kdgK51 79.26 1 kdgK51 xylA5 80.34 1 xylA5 mtl-1 81.26 1 mtl-1 argE3(Oc) 89.48 1 argE3(Oc) thiE1 90.34 1 thiE1 - Comments:
- This strain contains a plasmid encoding a temperature-sensitive Lambda repressor gene. It is commonly used for heat induction of Klenow fragment expression as described in the listed Joyce and Grindley, 1983 reference
- Episome/Plasmid: pCJ136
Plasmid Markers/Mutations: ori p15A [cI857](lambda)(ts) cat - References:
- Joyce, CM, ND Grindley 1983. Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 80(7):1830-4.
- Derbyshire, V, ND Grindley, CM Joyce 1991. The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction. EMBO J. 10(1):17-24.
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