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Strain - BW20767/pRL27Strain BW20767/pRL27Strain BW20767/pRL27
- CGSC#: 12551
- Strain Designation
- Sex(Hfr,F+,F-,or F'): F-
- 8 Mutations:
- Comments:
- This plasmid is known to be somewhat unstable, presumably because of constitutive expression of the of the transposase. As a result mutations in the tnp gene tend to accumulate and these mutants will not work for mutagenesis.
- It is suggested that you immediately test several colonies to verify their ability transfer the transposon. Once this is verified, freeze the strain immediately and minimize the passage of the strain to reduce the accumulation of tnp mutants.
- Episome/Plasmid: pRL27
| - References:
- Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
- Larsen, RA, MM Wilson, AM Guss, WW Metcalf 2002. Genetic analysis of pigment biosynthesis in Xanthobacter autotrophicus Py2 using a new, highly efficient transposon mutagenesis system that is functional in a wide variety of bacteria. Arch. Microbiol. 178(3):193-201.
