E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation RP4-2(Km::Tn7,Tc::Mu-1)

• Name: RP4-2(Km::Tn7,Tc::Mu-1)
• Type: Insertion
• Approx. Map Location: -1.00
• Properties

  Property	Symbol
  NULL	Tc::Mu-1
  NULL	Km::Tn7

  
  
• Comments
  • integrated plasmid is Kan and Tet sensitive, due to the insertions in Tc and Km, and the Tn7 results in TRIM/aminopterin resistance and low level strR.
  • The RP4-2 also has an ISR1 insertion element within the Tn1³⁵ transposon that inactivates the adjacent ampicillin resistance gene.
• References
• 6 Strains Carrying This Mutation

  Name	Mutations	Genotype
  BW19851	7	F-, RP4-2(Km::Tn7,Tc::Mu-1), ΔuidA3::pir+, recA1, endA1, thiE1, hsdR17, creC510
  BW20767	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), leu-163::IS10, ΔuidA3::pir+, recA1, endA1, thiE1, hsdR17, creC510
  BW20767/pRL27	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), leu-163::IS10, ΔuidA3::pir+, recA1, endA1, thiE1, hsdR17, creC510, pRL27
  S17-1 λpir/pLFT	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510, pLFT
  S17-1 λpir/pLFX	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510, pLFX
  S17-1 λpir/pUV5	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510, pUV5