E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Mutation leu-163::IS10

• Name: leu-163::IS10
• Type: Insertion
• Mutation of Gene: leu
• Approx. Map Location: 1.70
• Comments
  • This allele was created by selecting for a spontaneous TetS version of leu-63::Tn10
• References
  • Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
• 2 Strains Carrying This Mutation

  Name	Mutations	Genotype
  BW20767	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), leu-163::IS10, ΔuidA3::pir+, recA1, endA1, thiE1, hsdR17, creC510
  BW20767/pRL27	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), leu-163::IS10, ΔuidA3::pir+, recA1, endA1, thiE1, hsdR17, creC510, pRL27