E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain JF618

• CGSC#: 5566
• Strain Designation

  Designation	Source	Preference
  JF618	Friesen J.D.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 17 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  araC14	1.52	1	araC14
  leuB6(Am)	1.74	1	leuB6(Am)
  DE(gpt-proA)62	5.51	1	Δ(gpt-proA)62
  lacY1	7.79	1	lacY1
  glnX44(AS)	14.99	1	glnX44(AS)
  galK2(Oc)	16.99	1	galK2(Oc)
  LAM-	17.40	1	λ-
  hisG4(Oc)	45.01	1	hisG4(Oc)
  cdd-6	48.07	1	cdd-6
  pyrG51	62.64	1	pyrG51
  rpsL31(strR)	74.84	1	rpsL31(strR)
  xylA5	80.34	1	xylA5
  mtl-1	81.26	1	mtl-1
  pyrE60	82.19	1	pyrE60
  argE3(Oc)	89.48	1	argE3(Oc)
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • Note that this strain requires added cytidine, even in rich media.
• References:
  • Friesen, JD, J Parker, RJ Watson, NP Fill, S Pedersen, FS Pedersen 1976. Isolation of a lambda transducing bacteriophage carrying the relA gene of Escherichia coli. J. Bacteriol. 127(2):917-22.
  
  

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