E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain RP437

• CGSC#: 12122
• Strain Designation

  Designation	Source	Preference
  RP437	Parkinson J.S.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 15 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  araC14	1.52	1	araC14
  leuB6(Am)	1.74	1	leuB6(Am)
  fhuA31	3.61	1	fhuA31
  lacY1	7.79	1	lacY1
  tsx-78	9.28	1	tsx-78
  LAM-	17.40	1	λ-
  eda-50	41.61	1	eda-50
  hisG4(Oc)	45.01	1	hisG4(Oc)
  rfbC1	45.43	0	rfbC1
  rpsL136(strR)	74.84	1	rpsL136(strR)
  xylA5	80.34	1	xylA5
  mtl-1	81.26	1	mtl-1
  metF159(Am)	89.03	1	metF159(Am)
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • Advice from J.S.Parkinson: After reviving this strain from the filter, test on soft agar plates and pick a colony that is motile and chemotactic for your experiments, because nonmotile variants arise in room temperature stab cultures.
  • This is the parent strain of many che and mot mutants. For questions about use or characteristics of this strain, contact parkinson@biology.utah.edu.
• References:
  • Parkinson, JS 1978. Complementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis. J. Bacteriol. 135(1):45-53.
  
  

  Printable Strain Report