CGSC Strain#: 12122
Strain Designation: RP437      Source of Strain: J.S. Parkinson
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), fhuA31, lacY1, tsx-78, λ^-, eda-50, hisG4(Oc), rfbC1, rpsL136(strR), xylA5, mtl-1, metF159(Am), thiE1
Strain Comments:

• Advice from J.S.Parkinson: After reviving this strain from the filter, test on soft agar plates and pick a colony that is motile and chemotactic for your experiments, because nonmotile variants arise in room temperature stab cultures.
• This is the parent strain of many che and mot mutants. For questions about use or characteristics of this strain, contact parkinson@biology.utah.edu.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Parkinson, JS 1978. Complementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis. J. Bacteriol. 135(1):45-53.