E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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We have two standard "Gene Disruption Kits", a 5 strain kit containing just the basic plasmids, and an 8 strain kit containing additional strains that some researchers find useful. Use of these kits are described in this Datsenko & Wanner 2000 reference.

5 Strain Wanner Lambda Red Gene Disruption Kit

CGSC#	Name	Genotype
7739	BW25113/pKD46	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD46
7629	BT340	F-, Δ(argF-lac)169, φ80dlacZ58(M15), glnX44(AS), λ-, rfbC1, gyrA96(NalR), recA1, endA1, spoT1, thiE1, hsdR17, pCP20
7633	BW25141/pKD13	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD13
7631	BW25141/pKD3	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD3
7632	BW25141/pKD4	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD4

8 Strain Wanner Lambda Red Gene Disruption Kit

CGSC#	Name	Genotype
7636	BW25113	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514
7739	BW25113/pKD46	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD46
7629	BT340	F-, Δ(argF-lac)169, φ80dlacZ58(M15), glnX44(AS), λ-, rfbC1, gyrA96(NalR), recA1, endA1, spoT1, thiE1, hsdR17, pCP20
7635	BW25141	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514
7633	BW25141/pKD13	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD13
7631	BW25141/pKD3	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD3
7988	BW25141/pKD32	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD32
7632	BW25141/pKD4	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD4