Strain - BW25141/pKD13

Strain - BW25141/pKD13Strain BW25141/pKD13

Strain BW25141/pKD13

CGSC#: 7633
Strain Designation
Designation Source Preference
BW25141/pKD13 Wanner B.L. 1
pKD13 2
Sex(Hfr,F+,F-,or F'): F-

Designation	Source	Preference
BW25141/pKD13	Wanner B.L.	1
pKD13		2

11 Mutations:

Mutation	Location	Certainty	Display
DE(araD-araB)567	1.40	1	Δ(araD-araB)567
lacZ4787(del)(::rrnB-3)	7.81	1	ΔlacZ4787(::rrnB-3)
DE(phoB-phoR)580	8.96	1	Δ(phoB-phoR)580
LAM-	17.40	1	λ^-
galU95	27.82	1	galU95
uidA3(del)::pir+	36.48	1	ΔuidA3::pir⁺
recA1	60.80	1	recA1
endA9(del-ins)::FRT	66.57	1	endA9(del-ins)::FRT
rph-1	82.20	1	rph-1
DE(rhaD-rhaB)568	88.16	1	Δ(rhaD-rhaB)568
hsdR514	98.74	1	hsdR514

Comments:
- The rha and ara deletions were published as Δ rhaBAD_LD78 and Δ araBAD_AH33
- This strain should be grown on rich media with ampicillin (100 ug/ml), kanamycin (50 ug/ml) or both, at 37 C. pKD13 sequence: GenBank AY048744.
Episome/Plasmid: pKD13
Plasmid Markers/Mutations:
oriR6Kgamma
tL3LAM(Ter)
bla(ApR)
rgnB(Ter)
kan
bla
FRT
kan
[tL3]
References:
- Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.
To request this strain visit the ECGRC website.

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