E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Strain PA200 S^RF₁

• CGSC#: 5104
• Strain Designation

  Designation	Source	Preference
  PA200 SRF1	Jacob F.	1

  
  
• Sex(Hfr,F+,F-,or F'): F'
• 14 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  leuB6(Am)	1.74	1	leuB6(Am)
  fhuA2::IS2	3.61	1	fhuA2::IS2
  lacY1	7.79	1	lacY1
  glnX44(AS)	14.99	1	glnX44(AS)
  gal-6	16.96	1	gal-6
  LAM-	17.40	1	λ-
  hisG1	45.01	1	hisG1
  malT1(LamR)	76.54	1	malT1(λR)
  xyl-7	80.34	1	xyl-7
  mtlA2	81.26	1	mtlA2
  argH1(del)	89.55	1	ΔargH1
  rplL9(L?)	90.06	1	rplL9(L?)
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • F100 is the long F-gal; F1-gal.
• Episome/Plasmid: F100

  Plasmid Markers/Mutations:

• References:
  • Brenner, S., J.R. Beckwith 1965. Ochre mutants, a new class of suppressible nonsense mutants. J.Mol.Biol. 13:629-637
  • Ohtsubo, E, MT Hsu 1978. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. J. Bacteriol. 134(3):778-94.
  
  

  Printable Strain Report