E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain NH5054

• CGSC#: 5970
• Strain Designation

  Designation	Source	Preference
  NH5054	Howard-Flanders P.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 8 Mutations:

  Mutation	Location	Certainty	Display
  glnX44(AS)	14.99	1	glnX44(AS)
  LAM-	17.40	1	λ-
  uvrB5	17.52	1	uvrB5
  sbcB15	44.85	1	sbcB15
  recB21	63.60	1	recB21
  endA1	66.57	1	endA1
  thiE1	90.34	1	thiE1
  deo-27 or deo-43	99.00	1	deo-27 or deo-43

  
  
• Comments:
  • deo-27 is deoB or deoC. This strain is φX-sensitive but plates φX poorly with turbid plaques. This is an E. coli K-12 x C hybrid.
• References:
  • Cassuto, E, J Mursalim, P Howard-Flanders 1978. Homology-dependent cutting in trans of DNA in extracts of Escherichia coli: an approach to the enzymology of genetic recombination. Proc. Natl. Acad. Sci. U.S.A. 75(2):620-4.
  
  

  Printable Strain Report