CGSC Strain#: 5970
Strain Designation: NH5054      Source of Strain: P. Howard-Flanders
Sex: F- Chromosomal Markers: glnX44(AS), λ^-, uvrB5, sbcB15, recB21, endA1, thiE1, deo-27 or deo-43
Strain Comments:

• deo-27 is deoB or deoC. This strain is φX-sensitive but plates φX poorly with turbid plaques. This is an E. coli K-12 x C hybrid.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor

References:

• Cassuto, E, J Mursalim, P Howard-Flanders 1978. Homology-dependent cutting in trans of DNA in extracts of Escherichia coli: an approach to the enzymology of genetic recombination. Proc. Natl. Acad. Sci. U.S.A. 75(2):620-4.