E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain KL363

• CGSC#: 6209
• Strain Designation

  Designation	Source	Preference
  KL363	Morgan S.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 13 Mutations:

  Mutation	Location	Certainty	Display
  araC14	1.52	1	araC14
  lacY1	7.79	1	lacY1
  tsx-57	9.28	1	tsx-57
  glnX44(AS)	14.99	1	glnX44(AS)
  galK2(Oc)	16.99	1	galK2(Oc)
  recA1	60.80	1	recA1
  metC56	67.91	1	metC56
  rpsL69(strR)	74.84	1	rpsL69(strR)
  glnT4(ts)	77.95	1	glnT4(ts)
  xyl-7 or xylA5	80.30	1	xyl-7 or xylA5
  lldD1	81.42	0	lldD1
  thiE1	90.34	1	thiE1
  valS7(ts)	96.54	1	valS7(ts)

  
  
• Comments:
  • The mutation gltnT4 renders this strain temperature-sensitive.
• References:
  • Morgan, S., A. Korner, K.B. Low, D. Soll 1977. Regulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. I. Isolation and characterization of a mutant with elevated levels of tRNA_1^Gln. J.Mol.Biol. 117:1013-1031
  
  

  Printable Strain Report