E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain EM111

• CGSC#: 4177
• Strain Designation

  Designation	Source	Preference
  EM111	Murgola E.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 11 Mutations:

  Mutation	Location	Certainty	Display
  tsx-57	9.28	1	tsx-57
  glnX44(AS)	14.99	1	glnX44(AS)
  galK2(Oc)	16.99	1	galK2(Oc)
  rfbC1	45.43	1	rfbC1
  mgl-51	48.17	1	mgl-51
  rpsL20(strR)	74.84	1	rpsL20(strR)
  xyl-6	80.30	1	xyl-6
  mtlA2	81.26	1	mtlA2
  gltE2	81.45	1	gltE2
  thiE1	90.34	1	thiE1
  tfr-5	1000.00	1	tfr-5

  
  
• Comments:
  • This strain must be grown in the presence of 200 ug/ml streptomycin in order to prevent selection of faster-growing revertants of the streptomycin-suppressible lethal mutation gltE2.
• References:
  • Murgola, E., E.A. Adelberg 1970. Mutants of Escherichia coli K-12 with an altered glutamyl-transfer ribonucleic acid. J.Bacteriol. 103:178-183
  
  

  Printable Strain Report