E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain BW19851

• CGSC#: 8175
• Strain Designation

  Designation	Source	Preference
  BW19851	Wanner B.L.	1
  S17-1 -like Su-		8

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 7 Mutations:

  Mutation	Location	Certainty	Display
  RP4-2(Km::Tn7,Tc::Mu-1)	-1.00	1	RP4-2(Km::Tn7,Tc::Mu-1)
  uidA3(del)::pir+	36.48	1	ΔuidA3::pir+
  recA1	60.80	1	recA1
  endA1	66.57	1	endA1
  thiE1	90.34	1	thiE1
  hsdR17	98.74	1	hsdR17
  creC510	99.89	1	creC510

  
  
• Comments:
  • This strain differs from S17-1 in that it is suppressor-negative and carries pir as an insertion in the uidA gene rather than as λ pir.
• References:
  • Metcalf, W.W., W. Jiang, B.L. Wanner 1994. Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6Kgamma origin plasmids at different copy numbers. Gene 138:1-7
  
  

  Printable Strain Report