Strain - NM81

Strain - NM81Strain NM81

Strain NM81

CGSC#: 7972
Strain Designation
Designation Source Preference
NM81 Schuldiner S. 1
Sex(Hfr,F+,F-,or F'): F-

6 Mutations:

Mutation	Location	Certainty	Display
thr-1	0.01	1	thr-1
nhaA1(del)::kan	0.38	1	ΔnhaA1::kan
DE(lacY-lacZ)9998	7.77	1	Δ(lacY-lacZ)9998
rpsL-(strR)	74.84	1	rpsL-(strR)
melB-(ts,LiD)	93.57	1	melB-(ts,LiD)
zjd-2334::Tn10	93.70	1	zjd-2334::Tn10

Comments:
- To avoid sensitivity to Na⁺ at pH above 7.5 in this nhaA^- nhaB⁺ strain, LBK medium, which substitutes KCl (6.5 g/l) for NaCl in LB medium, may be used.
References:
- Padan, E., N. Maisler, D. Taglicht, R. Karpel, S. Schuldiner 1989. Deletion of ant in Escherichia coli reveals its function in adaptation to high salinity and an alternative Na+/H+ antiporter systems(s). J.Biol.Chem. 264:20297-20302
- Pinner, E., Y. Kotler, E. Padan, S. Schuldiner 1993. Physiological role of nhaB, a specific Na+/H+ antiporter in Escherichia coli. J.Biol.Chem. 268:1729-1734
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