Strain - HS2012

Strain - HS2012Strain HS2012

Strain HS2012

CGSC#: 6146
Strain Designation
Designation Source Preference
HS2012 1
Sex(Hfr,F+,F-,or F'): F-

11 Mutations:

Mutation	Location	Certainty	Display
araD139([B/r])	1.42	1	[araD139]_B/r
DE(argF-lac)169	6.21	1	Δ(argF-lac)169
LAM-	17.40	1	λ^-
flhD5301	42.59	1	flhD5301
DE(fruK-yeiR)725(fruA25)	48.70	1	Δ(fruK-yeiR)725(fruA25)
relA1	62.71	1	relA1
rpsL150(strR)	74.84	1	rpsL150(strR)
rpoB324(rifR)	90.08	1	rpoB324(rifR)
thiE1	90.34	0	thiE1
malF64(Oc)	91.42	1	malF64(Oc)
deoC1	99.48	1	deoC1

Comments:
- malF64 is referred to as malFv49 in the paper cited below. The thr- leu region was transduced in from E. coliB.
- Not a pure K-12 strain.
References:
- Silhavy, TJ, E Brickman, PJ Bassford, MJ Casadaban, HA Shuman, V Schwartz, L Guarente, M Schwartz, JR Beckwith 1979. Structure of the malB region in Escherichia coli K12. II. Genetic map of the malE,F,G operon. Mol. Gen. Genet. 174(3):249-59.
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