CGSC Strain#: 6146
Strain Designation: HS2012      Source of Strain:
Sex: F- Chromosomal Markers: [araD139]_B/r, Δ(argF-lac)169, λ^-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rpoB324(rifR), thiE1, malF64(Oc), deoC1
Strain Comments:

• malF64 is referred to as malFv49 in the paper cited below. The thr- leu region was transduced in from E. coliB.
• [araD139]_B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• flhD5301-- flagellar defect shown in Komeda and Iino
• Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
• thiE1 was formerly called thi-1
• fruA25 = former designation
• Oc = ochre(UAA) mutation
• rifR = rifamycin resistance
• strR = streptomycin resist.

References:

• Silhavy, TJ, E Brickman, PJ Bassford, MJ Casadaban, HA Shuman, V Schwartz, L Guarente, M Schwartz, JR Beckwith 1979. Structure of the malB region in Escherichia coli K12. II. Genetic map of the malE,F,G operon. Mol. Gen. Genet. 174(3):249-59.