E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain MG1655/pKD20

• CGSC#: 7668
• Strain Designation

  Designation	Source	Preference
  MG1655/pKD20	Wanner B.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 2 Mutations:

  Mutation	Location	Certainty	Display
  LAM-	17.40	1	λ-
  rph-1	82.20	1	rph-1

  
  
• Comments:
  • Grow this strain at 30C in presence of ampicillin (100ug/ml for rich media).
  • pKD20 sequence: GenBank AY048745
• Episome/Plasmid: pKD20

  Plasmid Markers/Mutations:
  repA101(ts)
  araBp-gam-bet-exo
  oriR101
  bla(ApR)
  araC
  bla

• References:
  • Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.
  
  

  Printable Strain Report