E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Strain JM70

• CGSC#: 5044
• Strain Designation

  Designation	Source	Preference
  JM70	Jones-Mortimer M.C.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 16 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  leuB6(Am)	1.74	1	leuB6(Am)
  fhuA2::IS2	3.61	1	fhuA2::IS2
  lacY1	7.79	1	lacY1
  glnX44(AS)	14.99	1	glnX44(AS)
  gal-6	16.96	1	gal-6
  LAM-	17.40	1	λ-
  trp-1	28.00	1	trp-1
  hisG1	45.01	1	hisG1
  rpsL99(strR)	74.84	1	rpsL99(strR)
  malT1(LamR)	76.54	1	malT1(λR)
  xyl-7	80.34	1	xyl-7
  mtlA2	81.26	1	mtlA2
  cysE52	81.47	1	cysE52
  argH1(del)	89.55	1	ΔargH1
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • This strain is sensitive to cysteine: use cystine instead.
• References:
  • Jones-Mortimer, MC 1968. Positive control of sulphate reduction in Escherichia coli. Isolation, characterization and mapping oc cysteineless mutants of E. coli K12. Biochem. J. 110(3):589-95.
  
  

  Printable Strain Report