Strain TKL10
- CGSC#: 5466
- Strain Designation
Designation Source Preference TKL10 Rorsch A. 1 817 2 H1159 2 PC 1243 2 - Sex(Hfr,F+,F-,or F'): F-
- 19 Mutations:
Mutation Location Certainty Display thr-1 0.01 1 thr-1 leuB6(Am) 1.74 1 leuB6(Am) fhuA21 3.61 1 fhuA21 codA1 7.66 1 codA1 lacY1 7.79 1 lacY1 tsx-95 9.28 1 tsx-95 glnX44(AS) 14.99 1 glnX44(AS) LAM- 17.40 1 λ- dadX21(ts) 26.69 0 dadX21(ts) dadX20 26.69 1 dadX20 trp-64 28.00 1 trp-64 pyrF101 28.88 1 pyrF101 his-108 44.00 1 his-108 thyA6 63.86 1 thyA6 argG66 71.48 1 argG66 ilvA634 85.21 1 ilvA634 thiE1 90.34 1 thiE1 alr-1(ts) 91.90 1 alr-1(ts) deoC1 99.48 1 deoC1 - Comments:
- This strain can grow on D-alanine and at 42 degrees it requires D-alanine supplementation. The strain originally carried an asp mutation, but has now lost its requirement for aspartic acid (H.J.W.Wijsman, personal communication).
- This strain does not use L-alanine as a carbon source at 28C or 42C.
- References:
- Wijsman, HJ 1972. The characterization of an alanine racemase mutant of Escherichia coli. Genet. Res. 20(3):269-77.
- Wasserman, S.A., C.T. Walsh, D. Botstein 1983. Two alanine racemase genes in Salmonella typhimurium that differ in structure and function. J.Bacteriol. 153:1439-1450
Printable Strain Report